Manual purification and extraction is a laborious process that includes repeated centrifugation and other mechanical or chemical treatment. Perkinelmer new coronavirus nucleic acid detection kit fda. Based on perkinelmer patented magnetic bead technology the chemagic 360 instrument represents the ideal solution for nucleic acid isolation in a huge variety of research market segments including but not limited to human genetics, biobanking, hla typing, virus and bacteria detection. Perkin elmer chemagic prepitod please refer to the chemagic prepitod instrument user manual revision 20220020 part number. Please follow chemagic 360 user manual for extraction setup. Preparing chemagic 360 plates for protease k, the internal standard, and polya beads. The chemagic dispenser 360 notably reduces handson time when preparing a chemagic 360 run. For ease of operation and advanced qa needs the chemagic software generates log. Due to potential contagious pathogens in human blood. The chemagic 360 instrument represents the ideal solution for nucleic acid isolation in a huge variety of research market segments including but not limited to biobankinghuman genetics, hla typing, virus and bacteria detection. Follow the chemagic 360 user manual to select the appropriate protocol. Rna extraction reagents and instrument the perkinelmer nucleic acid extraction kits kn0212 and prenat ii sy61 software version 1.
Janus chemagic 360 workstation from perkinelmer biocompare. Principle of manual nucleic acid isolation with magnetic beads. The modular set up of the system and convenient loading procedure allow its integration with standard liquid handling robots, such as perkinelmers janus or sciclone. The exemplary data from donor 2 shows that the yield of cfdna is scalable to sample input. Perkinelmer launches chemagic prime instrument for.
Experience the revolutionary compact benchtop design of our new developed chemagic 360 instrument. Perkinelmer launches chemagic prime instrument for automated. The kit generated robust cfdna recovery from both plasma and serum on the chemagic 360 and chemagic msm i platforms as analyzed with electropherogram traces and qubit spectrophotometer. Built with renown chemagen technology, the chemagic 360 offers the greatest flexibility when working with various starting materials blood, saliva plasma, sample volumes 10 l10 ml and throughputs 112, 124, 196 all within a small footprint, ensuring high quality dnarna applicable for sensitive dowmstream assays like ngs, mlpa and. Nucleic acid extractor chemagic 360 instrument medical. The use of the chemagic 360 instrument simplifies and improves time consuming sample preparation steps in nucleic acid isolation significantly, by using the chemagic dispenser 360 unit. Perkinelmers chemagic 360 instrument with the janus automated liquid handling system.
Equipped with barcodereader and qa software, the chemagic 360 instrument complies with lims compatibility and modern quality assurance standards. Instructions for perkinelmer sarscov2 nucleic acid. Sep 01, 2015 features at a glance sample volumes from 10 l 10 ml high throughput 1 96 samples per run revolutionary compact design 80 x 80 x 90 cm lwh integrates with liquid handling platforms compact high volume nucleic acid isolation experience the revolutionary compact benchtop design of our next generation instrument for dnarna isolation. Cmg5554 is designed for use with the chemagic 360 and prime instruments equipped with chemagic 24 rod head art. For different sample volumes please refer to the kit selection page. Adding beads and resuspension buffer to deep well plates for downstream rna extraction. The genomic analysis of large numbers of samples requires a reliable system for the automated isolation of dna and rna. Based on perkinelmer patented magnetic bead technology, chemagic automation instruments, with the tmprevious developed chemagic 360 instrument and the well established chemagic msm i instrument, are an ideal solution for isolating nucleic acid from a huge variety of sample materials. The chemagic 360 d instrument meets the requirements of the in vitro diagnostic medical device directive 9879ec.
This user friendly modular system benefits from more than 10 years experience with the previous wellreceived high throughput chemagic msm i instrument and facilitates high volume nucleic acid isolation within a compact benchtop system. The use of the chemagic 360 instrument simplifies and improves time consuming sample preparation steps in nucleic acid isolation significantly, by using the. Researchers specializing in biobanking, genetics and next generation sequencing can leverage the chemagic prime technology to streamline and automate high quality dna and rna extraction for a wide range of human samples. Janus g3 workstations for sarscov2 testing perkinelmer. Based on perkinelmer patented magnetic bead technology the chemagic 360 represents the ideal solution for nucleic acid isolation in a variety. Based on perkinelmer patented magnetic bead technology the chemagic 360d instrument represents the ideal solution for nucleic acid isolation in a wide range of laboratory applications. The chemagic 360 instrument represents the ideal solution for nucleic acid isolation in a huge variety of research market segments including but not limited to. Please find below a selection of available kits for viral dnarna isolation from low volumes of plasma or serum 50 l to 1 ml using chemagic instruments, liquid handling workstations, or a manual procedure. Automated dnarna isolation automated nucleic acid isolation. Fill out the perkinelmer run check list taped to the side of the machine.
Nucleic acid extractor for medium to high throughput. Extraction procedure with chemagic 360 please follow chemagic 360 user manual for extraction setup. Based on our patented chemagen magnetic bead technology the chemagic 360 instrument represents the ideal solution for nucleic acid isolation in a huge variety of research market segments including but not limited to biobankinghuman genetics, hla typing, virus and bacteria detection. Sarscov2 testing workflow perkinelmer for the better.
The chemagic prime instrument can transfer primary samples into the plates used for nucleic acid extraction, perform the entire procedure of dna and rna. Compact, fast, ready to use high quality dnarna based on perkinelmer patented magnetic bead technology the chemagic 360 represents the ideal solution for nucleic acid isolation from a variety of starting materials e. May 26, 2020 received fda eua amendment to add perkinelmers chemagic viral dna rna 300 kit h96 and chemagic 360 instrument to new coronavirus rtpcr test. Based on chemagens proprietary mpva magnetic beads technology for highyield extraction of ultrapure nucleic acids, the chemagic prepito instrument represents the optimal system for laboratories that process less than one hundred samples per day or need to add flexibility to their current high.
The chemagic 360 instrument represents the latest version of chemagic automation. The chemagic 360 instrument is an automated system that can be adapted to fit almost all of your dna and rna purification needs. Based on perkinelmers patented chemagen magnetic bead technology, the chemagic 360 instrument. Nextprepmag cfdna automated isolation kit perkinelmer.
Unique sample volume flexibility through exchangeable chemagic rod heads 1 12 samples a 1 ml 10 ml. Based on perkinelmer patented magnetic bead technology the chemagic 360d instrument represents the ideal solution for nucleic acid isolation in a wide. The chemagic dispenser 360 unit notably reduces handson time when preparing a chemagic 360 instrument. Perkinelmer nucleic acid extraction kit instructions for use. Its the optimal automated benchtop solution designed for seamless integration into highthroughput sarscov2 detection workflows. With dimensions of only 80 x 80 cm and its unique sample volume flexibility, the chemagic 360 is an innovative instrument that can be.
The intuitive chemagic software is the ideal solution for laboratories that require high quality assurance while they use the chemagic 360 instrument for nucleic acid isolation. The chemagic 360 d instrument is fda listed and if used in combination with. Low volume pathogen nucleic acid kits perkinelmer for the. For cfdna analysis, a short fragment 115 bp from a consensus sequence with abundant genomic alu repeats was amplified4.
The ctrc uses a perkinelmer chemagic 360 instrument. Independent from sample input and throughput, cfdna. Chemagic 360 high quality dnarna isolation youtube. Experience the revolutionary compact benchtop design of our new developed chemagic 360d instrument. The chemagic 360 system for automated dna and rna purification. If something appears abnormal, ask dan winder to inspect the machine. Instructions for perkinelmer new coronavirus nucleic acid. By using chemagens wellestablished mpva magnetic bead technology, the chemagic 360 instrument provides high yields of ultrapure nucleic acids suitable for a wide range of downstream applications such as ngs, mlpa, genotyping, and pcr.
The chemagic 360 instrument is a nucleic acid extractor for medium to high throughput laboratories that features a revolutionary compact benchtop design and offers a flexible solution for different sample processing and throughput needs. Load the magnetic rods disposable tips onto the tip rack according to the number of. In this technical note it is shown that the new chemagic cfdna kits used on the chemagic 360 instrument obtain similar yields compared to a manual competitor silica membrane based kit. Perkinelmer new coronavirus nucleic acid detection kit. Perkinelmer sarscov2 solutions for nucleic acid detection. Kit features optimized for the use with chemagic instruments for high and mediumthroughput nucleic acid isolation proprietary technology based on the use of. Based on perkinelmer patented magnetic bead technology the chemagic 360 represents the ideal solution for nucleic acid isolation in a variety of research mar. Kits for dna purification from different volumes of saliva the saliva chemagic kits for genomic dna purification are designed for the efficient isolation of highquality dna from 200. Perkinelmer chemagic 360, magnetic particle separator machine. The chemagic 360 nucleic acid purification instrument delivers transiently magnetized rods to attract the beads after binding to the nucleic acids. Perkinelmer chemagic 360d instrument community, manuals and. Catalog number janus chemagic 360 this product is no longer available on biocompare.
Jul 29, 2019 an example of this new trend is the perkinelmer chemagic prime instrument, integrating the janus g3 liquid handler with a chemagic 360 nucleic acid extraction instrument. Quick dna, rna, and protein isolation methods are necessary due to the natural degradation of the samples. Automated perkinelmer chemagic 360 protocol university of utah. The ce ivdregistered chemagic 360 d instrument is for distribution and use in specific european countries only. Automated circulating cellfree dna purification with the. Close the chemagic 360 safety cover on the perkinelmer machine. Instructions for perkinelmer sarscov2 nucleic acid detection. Highly reproducible cfdna extractions on chemagic 360 instrument. When the extraction run has finished, examine all racks and tubes for unusual problems. This solution helps address the serious bottleneck laboratories have been experiencing, as it provides rna extraction from the viral particle prior to rtpcr. With dimensions of only 80 x 80 cm and its unique sample volume flexibility. Do not touch any part of the machine during operation once the machine has started. The chemagic kits provide a reliable isolation of high yields of cfdna from 1 5 ml plasma per sample. This is the world s first plate with a capacity of 25 ml per well.
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